Sero-X: Technical Update Verticillium Wilt Management

This document summarizes the trial work demonstrating Sero-X's efficacy against Verticillium Dahliae.
It updates the 2021-22-23 long-term RCB field trial measuring DNA abundance as an inoculum indicator.
Those who are familiar with the earlier work may wish to;
Those with time challenges can;
Everyone else please start with the;

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TLDR: Sero-X Verticillium Wilt long term DNA Abundance RCB field trial

Sero-X: A Botanical Solution Sero-X, a pesticide derived from the Butterfly Pea plant, is registered with the APVMA to manage Verticillium dahliae - a devastating soil-borne fungus that causes wilt disease in cotton crops. Its also registered for the control or suppression of a range of insect pests, including green mirids, silver leaf white fly (biotype b), heliothis, diamondback moth and two spotted spider mite, in cotton, lucerne, brassicas, cucurbits and tomatoes as specified in the "Directions for Use" table. Proven Efficacy Through extensive replicated trials conducted by Dr. Karen Kirkby and her team at the Australian Cotton Research Institute, Sero-X has demonstrated the remarkable ability to inhibit the formation of microsclerotia - the key survival structures of the Verticillium fungus. Managing Inoculum Levels Sero-X can either limit the increase of fungal inoculum in the soil or accelerate its decline, depending on seasonal conditions. This makes it a powerful tool for cotton growers to manage Verticillium wilt. A new Diagnostic tool DNA abundance testing (PREDICTA B) proved effective for measuring Sero-X's impact, in place of labour-intensive dry plating propagule counting Efficacy All Sero-X treatments reduced Verticillium dahliae DNA levels compared to untreated controls. LT 1 SD2....SD4 Label rate (2L/ha × 3) 62% in-season exposure reduction Half label rate (1L/ha × 3) 42% in-season exposure reduction Lowest rate (500mL/ha × 2 + 1L/ha at defoliation) 39% in-season exposure reduction Defoliation only (2L/ha × 1) 21% in-season exposure reduction Label rate for Tech review Integrated approach: While Sero-X significantly reduces risk during the cotton season, combining it with practices like fallow management offers a robust disease management strategy. Success against Verticillium Wilt. The trial successfully demonstrated Sero-X's effectiveness in inhibiting Verticillium Wilt microsclerotia, with higher application rates showing the best results. DNA testing As a diagnostic tool proved useful but had some limitations in specificity compared to traditional methods. Spatial variability While efforts to manage spatial variabilit across the field were challenging, adjustments to the sampling method improved data reliability. Silver bullets? While Sero-X significantly reduces risk during the cotton season, combining it with practices like fallow management offers a robust disease management strategy.